Metabolismo de zinco em Paracoccidioides bradiliensis

AUTOR(ES)

Nathalie Martelli de Paula

DATA DE PUBLICAÇÃO

2009

RESUMO

The thermally dimorphic fungus Paracoccidioides brasiliensis is the agent responsible for causing paracoccidioidomycosis, a common systemic mycosis in Latin America. The infectious process is characterized by the fungus conversion from a non-pathogenic to a pathogenic form. The infectious process establishment depends on the acquisition of essential micronutrients such as zinc, iron and copper, which are required as cofactors in several biological processes such as respiration, fungal growth and protein maturation. Previous studies developed in our laboratory showed the genes coding for high (PbZrt1) and low (PbZrt2) affinity iron and zinc transporters are induced during the infective process caused by P. brasiliensis in animal model. In the present study in silico analysis was performed on the database of the structural genome of P. brasiliensis (http://www.broad.mit.edu/annotation/genome/paracoccidioides_brasiliensis/MultiHome.html). Genes involved in maintaining the homeostasis of zinc have been identified and used for the development of a probable model of zinc homeostasis in P. brasiliensis. The technique of real time qRT-PCR was used to evaluate the expression profile of Pbzrt1 and Pbzrt2 and the probable genes involved in zinc homeostasis during exposure of yeast cells of P. brasiliensis to different concentrations of ZnSO4, as well as in absence of zinc, iron and copper. Real time qRT-PCR was also used to analyze the expression of Pbzrt1 and Pbzrt2 in yeast cells of P. brasiliensis derived from spleen of infected mice. All together data of this study suggest the relationship between zinc and iron ions in the homeostasis of zinc and the importance of high-affinity transporter of iron and zinc during the infectious process.

ASSUNTO(S)

transporte de ferro e zinco zinc homeostasis biologia molecular paracoccidioides brasiliensis homeostase de zinco paracoccidioides brasiliensis zinc and iron transport

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