Method Detection Limits of PCR and Immunofluorescence Assay for Cryptosporidium parvum in Soil

AUTOR(ES)

Walker, Mark J.

FONTE

American Society for Microbiology

RESUMO

We determined and compared the method detection limits (MDLα) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDLα and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport.

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