Method validation and stability study of quercetin in topical emulsions

AUTOR(ES)

Casagrande, Rúbia, Baracat, Marcela M., Georgetti, Sandra R., Verri Jr, Waldiceu A., Vicentini, Fabiana T. M. C., Rafael, Janice A., Jabor, José R., Fonseca, Maria J. V.

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2009

RESUMO

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

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