Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs.
AUTOR(ES)
Wang, R Y
RESUMO
Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP indicate that m5CpG is present in the recognition sites for this protein but is only part of the recognition sequence. Specific binding to MDBP was observed for bacteriophage XP12 DNA, which naturally contains approximately 1/3 of its residues as m5C, and for Micrococcus luteus DNA, M13mp8 replicative form (RF) DNA, and pBR322 when these three DNAs were methylated at CpG sites by human DNA methyltransferase. Five DNA regions binding to MDBP have been localized by DNase I footprinting or restriction mapping in methylated pBR322 and M13mp8 RF DNAs. A comparison of their sequences reveals a common 5'-m5CGRm5CG-3' element or closely related sequence in which one of the m5C residues may be replaced by a T. In addition to this motif, one upstream and one downstream m5CpG as well as other common residues over an approximately 20-bp long region may be recognized by MDBP.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341339Documentos Relacionados
- Related sites in human and herpesvirus DNA recognized by methylated DNA-binding protein from human placenta.
- Effect of site-specific DNA methylation and mutagenesis on recognition by methylated DNA-binding protein from human placenta.
- Highly repeated sites in the apolipoprotein(a) gene recognized by methylated DNA-binding protein, a sequence-specific DNA-binding protein.
- Methylated DNA-Binding Proteins from Arabidopsis1
- Using electrostatic potentials to predict DNA-binding sites on DNA-binding proteins