Methylation of Cytosine Residues in DNA Controlled by a Drug Resistance Factor

AUTOR(ES)

Hattman, Stanley

RESUMO

The proportion of 5-methylcytosine (5MeCyt) and 6-methylaminopurine (N6-methyladenine, 6MeAde) in bacteriophage P22 DNA was analyzed as a function of the host-specificity the phage carried. In the DNA of P22 grown in strains harboring the modifying drug-resistance-transfer-factor N-3, the 5MeCyt content was at least twice that after growth in strains lacking the factor. In contrast, the 6MeAde level of P22 DNA was unaffected by the presence or absence of the factor. The 6MeAde and 5MeCyt levels were unaffected by factors 222 and N-1, which do not modify phage DNA.

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