Microbial uptake of radiolabeled substrates: estimates of growth rates from time course measurements.

AUTOR(ES)

Li, W K

RESUMO

The uptake of [3H]glucose and a mixture of 3H-labeled amino acids was measured, in time course fashion, in planktonic microbial assemblages of the eastern tropical Pacific Ocean. The average generation times of those portions of the assemblages able to utilize these substrates were estimated from a simple exponential growth model. Other workers have independently used this model in its integrated or differential form. A mathematical verification and an experimental demonstration of the equivalence of the two approaches are presented. A study was made of the size distribution of heterotrophic activity, using time course measurements. It was found that the size distribution and the effect of sample filtration before radiolabeling were dependent on time of incubation. In principle, it was possible to ascribe these time dependences to differences in the specific growth rate and initial standing stock of the microbial assemblages.

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