Microimmunoassay Using a Protein Chip: Optimizing Conditions for Protein Immobilization
AUTOR(ES)
Seong, Seung-Yong
FONTE
American Society for Microbiology
RESUMO
Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=120026Documentos Relacionados
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