Microinjected simian virus 40 cRNA is spliced, as evidenced by electron microscopy.
AUTOR(ES)
Graessmann, M
RESUMO
Simian virus 40 cRNA was transcribed in vitro from the early viral DNA strand. The RNA was injected through glass capillaries into the nuclei of monkey cells. After a 2-h incubation, the RNAs were extracted and hybridized to single-stranded simian virus 40 DNA sequences contained in a bacteriophage M13 vector. Electron microscopy revealed processed cRNAs with splice loops in the region of the intron of large T antigen.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255345Documentos Relacionados
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