Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes.
AUTOR(ES)
de Wit, T
RESUMO
We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147291Documentos Relacionados
- Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase.
- Cre recombinase-mediated site-specific recombination between plant chromosomes.
- Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase.
- Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.
- Cre-mediated somatic site-specific recombination in mice.