Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci.
AUTOR(ES)
Orberg, P K
RESUMO
A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239747Documentos Relacionados
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