Microsporidia are related to Fungi: Evidence from the largest subunit of RNA polymerase II and other proteins
AUTOR(ES)
Hirt, Robert P.
FONTE
The National Academy of Sciences
RESUMO
We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our reexamination of elongation factors EF-1α and EF-2 sequence data that had previously been taken as support for an early (Archezoan) divergence of these amitochondriate protists show such support to be weak and likely caused by artifacts in phylogenetic analyses. These EF data sets are, in fact, not inconsistent with a Microsporidia + Fungi relationship. In addition, we show that none of these proteins strongly support a deep divergence of Parabasalia and Metamonada, the other amitochondriate protist groups currently thought to compose early branches. Thus, the phylogenetic placement among eukaryotes for these protist taxa is in need of further critical examination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=15179Documentos Relacionados
- The Largest Subunit of Human RNA Polymerase III Is Closely Related to the Largest Subunit of Yeast and Trypanosome RNA Polymerase III
- The second largest subunit of RNA polymerase II from Arabidopsis thaliana.
- Complete sequence of the human RNA polymerase II largest subunit.
- A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase II.
- The C-terminal domain of the largest subunit of RNA polymerase II and transcription initiation.