Mineralização do esmalte apos a remoção parcial dos ameloblastos pos-secretorios em incisivos inferiores de ratos / Enamel mineralization after partial removal of post-secretory ameloblasts in the rat lower incisors

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The enamel organ of rat incisors at the maturation stage of amelogenesis (from the region were enamel rods are visible and the enamel is completely acid-soluble) was removed and the limits of the removal (surgical region) were marked by notches made at the labial face of the enamel. The enamel organ, from the incisal notch up to the alveolar crest, was damaged by an endodontic file and the odontogenic organ at the basal end of the incisor was resected. The rats were divided into two groups: I ? when the incisal mark and II when the basal mark appeared at the clinical crown. The experimental incisors erupted at a variable and slower rate than the contralateral controls. The histological aspect of the enamel at the surgical region of rats of group I varied from enamel completely removed by demineralization to remaining of enamel showing rods. In the same region of group II all enamel was completely acid-soluble. Microhardness of the enamel at the surgical region was lower in experimental incisors, while the mineral content (Ca and P) did not differ from the control teeth. Protein analysis of enamel from incisors, one week after surgery, showed an amount and an electrophoresis pattern similar in experimental and control teeth at the surgical region. In the post-surgical region, the protein content of enamel of experimental teeth was much lower and showed more proteins of lower molecular weight than the correspondent region of the control incisors. The results are consistent with the hypothesis that the ameloblast at the maturation stage do not interfere with the influx of minerals into the enamel

ASSUNTO(S)

ameloblasts enamel ameloblastos esmalte

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