Minimal Requirements for Commitment to Sporulation in Bacillus megaterium
AUTOR(ES)
Greene, Richard A.
RESUMO
Commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. In both cases, a point of commitment to the completion of sporulation occurred at stage II. With 2 × 108 cells/ml, the process by which commitment occurred required 0.5 mm Mg2+, 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). For completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm Ca2+ was required. Except for this Ca2+ requirement, the completion of sporulation after commitment was independent of the nature of the surrounding medium. Some aspects of the utilization of these required substances were examined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251318Documentos Relacionados
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