Mispair formation in DNA can involve rare tautomeric forms in the template.
AUTOR(ES)
Strazewski, P
RESUMO
The formation of pyridine-pyrimidine- and pyrimidine-pyrimidine base pairs after in vitro DNA replication with the large fragment of Escherichia coli DNA polymerase I indicates that Watson-Crick-like base pairing between pyrimidine bases can occur in the enzyme due to the presence of the rare tautomers of deoxycytidylate and thymidylate in the template strand. The implications to mispair formation in DNA, such as the difference between the structures of the mispairs during and after replication, are discussed and the possible action of mutagenic DNA protonating and deprotonating agents in vivo is considered.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338751Documentos Relacionados
- Template functions in the enzymic formation of polyribonucleotides. IV. Denatured DNA as template.
- Template functions in the enzymic formation of polyribonucleotides, I. Integrity of the DNA template.
- Template functions in the enzymic formation of polyribonucleotides. 3. Apurinic acid as template.
- Solid phase in vitro mutagenesis using plasmid DNA template.
- Yeast RNA polymerase II initiates transcription in vitro at TATA sequences proximal to potential non-B forms of the DNA template.