Mode of action of a new type of UDP-glucose analog against herpesvirus replication.
AUTOR(ES)
Alarcón, B
RESUMO
The mode of action of a new type of UDP-glucose analog against herpes simplex virus type 1 (HSV-1) replication was examined. The analog showed good selectivity and potent activity. At 10 micrograms/ml, P-536 inhibited the formation of infectious HSV-1 by more than 90%, whereas at 100 micrograms/ml it had no cytotoxic effects, as evidenced by phase-contrast microscopy. P-536 showed a wide spectrum of action and was active against HSV-1, adenovirus type 5, vaccinia virus, poliovirus type 1, encephalomyocarditis virus, vesicular stomatitis virus, influenza virus, and measles virus, irrespective of whether these viruses have lipidic envelopes or not. P-536 clearly inhibited protein glycosylation if added at the time when late viral proteins were being synthesized. Moreover, it also interfered with the synthesis of nucleic acids and the phosphorylation of nucleosides. If P-536 was present from the beginning of infection, HSV-1 replication was blocked at an early step and the infected cells continued to synthesize cellular proteins for long periods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=172388Documentos Relacionados
- Activity of P536, a UDP-glucose analog, against Trypanosoma cruzi.
- UDP-Glucose Pyrophosphorylase. An Old Protein with New Tricks1
- Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases
- Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA
- Rapid Increase in UDP-Glucose during Early Wheat Embryo Germination 1