Mode of peptidoglycan synthesis in Salmonella typhimurium: single-strand insertion.

AUTOR(ES)

Cooper, S

RESUMO

The synthesis of peptidoglycan by Salmonella typhimurium at the molecular level has been analyzed by studying the pattern of insertion of newly synthesized strands into the preexisting cell wall. We have measured the acceptor-donor radioactivity ratio during short labeling periods, and we found values between 0 and 0.2. This is less than the ratio observed by Burman and Park (Proc. Natl. Acad. Sci. USA, 81:1844-1848) for peptidoglycan synthesis in Escherichia coli. We propose that insertion of new strands occurs as single strands.

Documentos Relacionados

• Single-strand binding protein enhances fidelity of DNA synthesis in vitro.
• Peptidoglycan synthesis during the cell cycle of Escherichia coli: composition and mode of insertion.
• Single-strand interruptions in replicating chromosomes cause double-strand breaks
• Replication of Bacteriophage M 13: Inhibition of Single-Strand DNA Synthesis by Rifampicin
• Mismatch cleavage by single-strand specific nucleases