Modeling the control of DNA replication in fission yeast
AUTOR(ES)
Novak, Bela
FONTE
The National Academy of Sciences of the USA
RESUMO
A central event in the eukaryotic cell cycle is the decision to commence DNA replication (S phase). Strict controls normally operate to prevent repeated rounds of DNA replication without intervening mitoses (“endoreplication”) or initiation of mitosis before DNA is fully replicated (“mitotic catastrophe”). Some of the genetic interactions involved in these controls have recently been identified in yeast. From this evidence we propose a molecular mechanism of “Start” control in Schizosaccharomyces pombe. Using established principles of biochemical kinetics, we compare the properties of this model in detail with the observed behavior of various mutant strains of fission yeast: wee1− (size control at Start), cdc13Δ and rum1OP (endoreplication), and wee1− rum1Δ (rapid division cycles of diminishing cell size). We discuss essential features of the mechanism that are responsible for characteristic properties of Start control in fission yeast, to expose our proposal to crucial experimental tests.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=23080Documentos Relacionados
- Meiotic DNA replication checkpoint control in fission yeast
- Complex mechanism of site-specific DNA replication termination in fission yeast
- Regulation of replication timing in fission yeast
- A key role for replication factor C in DNA replication checkpoint function in fission yeast.
- A Novel Intermediate in Initiation Complex Assembly for Fission Yeast DNA Replication