Modified Colorimetric Assay for Susceptibility Testing of Azole Antifungal Drugs against Candida Species
AUTOR(ES)
Chen, Jian
FONTE
American Society for Microbiology
RESUMO
We modified a rapid susceptibility assay (RSA) for antifungal susceptibility testing of azoles based upon glucose utilization. This modified RSA method provides quantitative endpoint readings in 6 h. In this study, the modified RSA and the National Committee for Clinical Laboratory Standards M27-A methods were used to determine the MICs of fluconazole and itraconazole for 118 Candida isolates. Yeast nitrogen base containing 0.12 g of glucose per liter was used for the modified RSA method. For fluconazole, agreement among assays within one or two twofold dilutions was 72.9 and 88.1%, respectively; for itraconazole, agreement within one or two twofold dilutions was 82.2 and 89.8%, respectively. These data suggest that the modified RSA method is a reliable and rapid method for azole antifungal susceptibility testing against Candida species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387539Documentos Relacionados
- Colorimetric Assay for Antifungal Susceptibility Testing of Aspergillus Species
- Improved method for azole antifungal susceptibility testing.
- Impact of Antifungal Prophylaxis on Colonization and Azole Susceptibility of Candida Species▿
- Modified agar dilution susceptibility testing method for determining in vitro activities of antifungal agents, including azole compounds.
- Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa
