Modified procedure for the recovery of naturally accumulated poliovirus from oysters.

AUTOR(ES)

Vaughn, J M

RESUMO

Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters.

Documentos Relacionados

• Methods for recovering poliovirus and rotavirus from oysters.
• Inefficient accumulation of low levels of monodispersed and feces-associated poliovirus in oysters.
• Improved methods for detecting enteric viruses in oysters.
• Recovery of viruses from water by a modified flocculation procedure for second-step concentration.
• Enzyme-capture assay for rapid detection of Escherichia coli in oysters.