Modifying Culture Conditions in Chemical Library Screening Identifies Alternative Inhibitors of Mycobacteria▿

AUTOR(ES)

Miller, Christopher H.

FONTE

American Society for Microbiology (ASM)

RESUMO

In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.

Documentos Relacionados

• New Approach for Drug Susceptibility Testing: Monitoring the Stress Response of Mycobacteria▿
• Quantitative specificity-based display library screening identifies determinants of antibody-epitope binding specificity
• Phosphate Uptake in an Obligately Marine Fungus II. Role of Culture Conditions, Energy Sources, and Inhibitors
• Cross-Reactive Immunity to Mycobacterium tuberculosis DosR Regulon-Encoded Antigens in Individuals Infected with Environmental, Nontuberculous Mycobacteria▿ †
• Inhibitors of Leishmania mexicana CRK3 Cyclin-Dependent Kinase: Chemical Library Screen and Antileishmanial Activity