Modulação da apresentação antigênica por células dendríticas derivadas de monócitos utilizando diferentes produtos virais do HIV: potencial de utilização em vacina terapêutica. / Modulation of monocyte-derived dendritic cell antigen presentation using different HIV antigenic: potential use in therapeutic vaccine.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Despite more than 20 years of effort, the design of an effective HIV-1 vaccine remains an enormous challenge. In this scenario, new immunological approaches must be considered. Monocytes from HIV-serodiscordant couples and non HIV exposed controls were differentiated in vitro into dendritic cells (MoDC), pulsed with different virus antigens and cultured with autologous lymphocytes. The T lymphocyte immunological response and the MoDC phenotype and function were determined. MoDCs were shown to be fully matured and activated in all antigen-pulsing protocols. Lymphocytes stimulated by DC pulsed with inactivated HIV or p55Gag protein showed greater activation, proliferation and IFNγ production in comparison to those stimulated by non pulsed MoDC. Nonetheless, T cells from non-exposed controls elicited the same response, except for the IFNγ production. This MoDC vaccine model may represent a viable and promising alternative of therapeutic vaccination against HIV.

ASSUNTO(S)

hiv vetores viral antigens vaccines vírus viruses dendritic cells vectors vacinas células dendríticas hiv antígenos de vírus

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