Modulation of Innate Cytokine Responses by Products of Helicobacter pylori
AUTOR(ES)
Meyer, Frank
FONTE
American Society for Microbiology
RESUMO
The gastric inflammatory and immune response in Helicobacter pylori infection may be due to the effect of different H. pylori products on innate immune mechanisms. The aim of this study was to determine whether bacterial components could modulate cytokine production in vitro and thus contribute to Th1 polarization of the gastric immune response observed in vivo. The effect of H. pylori recombinant urease, bacterial lysate, intact bacteria, and bacterial DNA on proliferation and cytokine production by peripheral blood mononuclear cells (PBMCs) from H. pylori-negative donors was examined as a model for innate cytokine responses. Each of the different H. pylori preparations induced gamma interferon (IFN-γ) and interleukin-12p40 (IL-12p40), but not IL-2 or IL-5, production, and all but H. pylori DNA stimulated release of IL-10. Addition of anti-IL-12 antibody to cultures partially inhibited IFN-γ production. In addition, each bacterial product inhibited mitogen-stimulated IL-2 production by PBMCs and Jurkat T cells. The inhibitory effect of bacterial products on IL-2 production correlated with inhibition of mitogen-stimulated lymphocyte proliferation, although urease inhibited IL-2 production without inhibiting proliferation, suggesting that inhibition of IL-2 production alone is not sufficient to inhibit lymphocyte proliferation. The results of these studies demonstrate that Th1 polarization of the gastric immune response may be due in part to the direct effects of multiple different H. pylori components that enhance IFN-γ and IL-12 production while inhibiting both IL-2 production and cell proliferation that may be necessary for Th2 responses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=97708Documentos Relacionados
- Decreased Epithelial Cytokine Responses in the Duodenal Mucosa of Helicobacter pylori-Infected Duodenal Ulcer Patients
- Induction of Maturation and Cytokine Release of Human Dendritic Cells by Helicobacter pylori
- Water-induced modulation of Helicobacter pylori virulence properties
- Modulation of Viral Immunoinflammatory Responses with Cytokine DNA Administered by Different Routes
- Local Cytokine Response in Helicobacter pylori-Infected Subjects