Moesin Is Not a Receptor for Measles Virus Entry into Mouse Embryonic Stem Cells

AUTOR(ES)

Doi, Yoshinori

FONTE

American Society for Microbiology

RESUMO

The involvement of moesin in measles virus (MV) entry was investigated with moesin-positive and -negative mouse embryonic stem (ES) cells. MV infection of these cells was very ineffective and was independent of moesin expression. Furthermore, when these cells were transfected to express human CD46, a 100-fold increase in syncytium formation was observed with these cells and was independent of the expression of moesin. The only obvious difference between moesin-positive and -negative ES cells was the shape of the syncytia formed. Moesin-negative ES cells expressing or not expressing human CD46 formed separate pieces of fragmented syncytia which were torn apart during spreading, whereas ES cells expressing moesin exhibited typical syncytia. In addition, moesin was not detected on the surface of any murine cells or cell lines that we have tested by a flow cytometric assay with moesin-specific antibodies. These findings indicate that murine moesin is neither a receptor nor a CD46 coreceptor for MV entry into mouse ES cells. Moesin is involved in actin filament-plasma membrane interactions as a cross-linker, and it affects only the spreading and shape of MV-mediated syncytia.

Documentos Relacionados

• Physical association of moesin and CD46 as a receptor complex for measles virus.
• CXC-Chemokine Receptor 4 Is Not a Coreceptor for Human Herpesvirus 7 Entry into CD4+ T Cells
• Mouse transferrin receptor 1 is the cell entry receptor for mouse mammary tumor virus
• The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
• A method for high efficiency YAC lipofection into murine embryonic stem cells.