Molecular characterization of arboviruses isolated from mosquitoes fauna (Diptera: nematocera) Rondonia state (western brazilian Amazon). / Caracterização molecular de arbovírus isolados da fauna diptera nematocera do Estado de Rondônia (Amazônia ocidental brasileira).

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The Rondônia state has an area with rich arthropods diversity although the knowledge about the arboviruses transmition for these species is poor. The present work aimed to detect arboviruses through RT-PRC and RT-PCR Duplex in the diptera species collected in the region as well as their characterization through the sequence reaction. The RT-PRC and RT-PCR Duplex detected the Mayaro and Oropouche virus suspensions until 104 e 101 TCID50/mL respectively, although it was negative for the Dengue 2 virus in pools containing less than three infected mosquitoes. The endogenous control was detected in 66,8 % of samples and from pools containing from one to three mosquitoes the detection rate was approximately half from that obtained from pools with 11 to 15 mosquitoes. Oropouche virus was found in 0,66 % of pools and Cacipacore virus also in 0,66 % of pools. Oropouche virus was detected in Coquillettidia sp. and Deinocerites sp. while Cacipacoré virus was found in Anopheles sp. and Culex sp. The techniques allowed the detection of examined arboviruses in the pools collected from Rondonia.

ASSUNTO(S)

flaviviruses diptera flavivirus diptera mosquitos rt-pcr arbovírus arboviruses rt_pcr culicidae culicidae mosquitoes

ACESSO AO ARTIGO

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