Molecular characterization of isolated ancestries of Bacillus sporothermodurans of milk UHT. / Caracterização molecular de linhagens de Bacillus sporothermodurans isoladas de leite UHT.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Aerobic mesophilic bacteria that form highly heat-resistant spores were first detected in UHT milk in southern Europe in 1985. The new species was named Bacillus sporothermodurans, and shown to be phylogenetically related to Bacillus megaterium. The identification of B. sporothermodurans is problematic, pushing the attempts for rapid and reliable methods for its detection and identification in raw and UHT milk. This need resulted in a PCR protocol, developed by Herman et al. (1997), named HRS-PCR. Typing of isolates in this species is also necessary, being useful for tracking the dissemination and contamination of products. The current study aimed at evaluating the genetic diversity of Bacillus sporothermodurans isolates originated from UHT milk samples from different regions of Brazil, using BOX-, ERIC- and REP-PCR fingerprinting. Results revealed a wide variation of banding patterns among the studied isolates. From 39 strains tested, 30 Brazilian isolates showed significant differences among rep-PCR patterns. These were more related amongst each other rather the European strains reference. The data demonstrated a high heterogeneity amongst cultures isolated from UHT milk in Brazil. The differentiation of Brazilian and European strains suggests that contamination problems found in Brazil are neither related to European isolates nor to the contamination of Brazilian processing plants by contaminants present in imported powdered-milk.

ASSUNTO(S)

leite milk uht rep-pcr bacillus sporothermodurans

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