Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene.

AUTOR(ES)

Larkin, J C

RESUMO

Using cloned DNA from the vicinity of the yeast mating type locus (MAT) as a probe, the wild type allele of the cryptopleurine resistance gene CRY1 has been isolated by the technique of chromosome walking and has been shown to be identical to the gene for ribosomal protein 59. A recessive cryR1 allele has also been cloned, using the integration excision method. The genetic distance from MAT to CRY1 is 2.2 cM, while the physical distance is 21 kb, giving a ratio of about 10 kb/cM for this interval. The phenotypic expression of both plasmid borne alleles of the gene can be detected in vivo. The use of this gene as a hybridization probe to examine RNA processing defects in the rna 2, rna 3, rna 4, rna 8, and rna 11 mutants is also discussed.

Documentos Relacionados

• Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.
• The ovalbumin gene: cloning and molecular organization of the entire natural gene.
• Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene.
• Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29.
• Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene.