Molecular cloning and biological characterization of the recA gene from Pseudomonas syringae.
AUTOR(ES)
Hickman, M J
RESUMO
We have identified a recombinant plasmid, pCUV8, from a cosmid library of Pseudomonas syringae genomic DNA which contains a functional analog of the Escherichia coli recA gene. The plasmid was initially identified by its ability to restore UV resistance to E. coli HB101. Quantitative analysis demonstrated that it restored both recombination proficiency and UV resistance to an E. coli recA deletion mutant. By these criteria, pCUV8 appears to contain the P. syringae recA gene. Several pathogenic and epiphytic strains of P. syringae, but not E. coli, showed sequence homology to pCUV8 under normal stringency.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=212212Documentos Relacionados
- Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO.
- Molecular cloning and characterization of the recA gene of Rhizobium phaseoli and construction of recA mutants.
- Cloning, Sequencing, and Characterization of the recA Gene from Rhodopseudomonas viridis and Construction of a recA Strain
- Molecular cloning and characterization of the recA gene from the cyanobacterium Synechococcus sp. strain PCC 7002.
- Molecular cloning of the recA gene and construction of a recA strain of Francisella novicida.