Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
AUTOR(ES)
Pines, J
RESUMO
We have isolated a cDNA clone encoding sea urchin cyclin and determined its sequence. It contains a single open reading frame of 409 amino acids which shows homology with clam cyclins. RNA transcribed in vitro from this sequence was efficiently translated in reticulocyte lysates, yielding full-length cyclin. Injection of nanogram amounts of this synthetic mRNA into Xenopus oocytes caused them to mature more rapidly than with progesterone treatment. The sea urchin cyclin underwent two posttranslational modifications in the Xenopus oocytes during maturation. The first occurred at about the time that maturation became cycloheximide-resistant, when a small apparent increase in the molecular weight of cyclin was observed. The second modification involved destruction of the cyclin at about the time of white spot appearance, just as would have occurred at the metaphase/anaphase transition in the natural environment of a cleaving sea urchin embryo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553735Documentos Relacionados
- Molecular cloning of five individual stage- and tissue-specific mRNA sequences from sea urchin pluteus embryos.
- Fertilization triggers unmasking of maternal mRNAs in sea urchin eggs.
- Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.
- Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
- Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.
