Molecular cloning and functional characterization of human vesicular glutamate transporter 3
AUTOR(ES)
Takamori, Shigeo
FONTE
Oxford University Press
RESUMO
Glutamate is the major excitatory neurotransmitter in the mammalian CNS. It is loaded into synaptic vesicles by a proton gradient-dependent uptake system and is released by exocytosis upon stimulation. Recently, two mammalian isoforms of a vesicular glutamate transporter, VGLUT1 and VGLUT2, have been identified, the expression of which enables quantal release of glutamate from glutamatergic neurons. Here, we report a novel isoform of a human vesicular glutamate transporter (hVGLUT3). The predicted amino acid sequence of hVGLUT3 shows 72% identity to both hVGLUT1 and hVGLUT2. hVGLUT3 functions as a vesicular glutamate transporter with similar properties to the other isoforms when it is heterologously expressed in a neuroendocrine cell line. Although mammalian VGLUT1 and VGLUT2 exhibit a complementary expression pattern covering all glutamatergic pathways in the CNS, expression of hVGLUT3 overlaps with them in some brain areas, suggesting molecular diversity that may account for physiological heterogeneity in glutamatergic synapses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1084213Documentos Relacionados
- Molecular cloning, chromosomal mapping, and functional expression of human brain glutamate receptors.
- The identification of vesicular glutamate transporter 3 suggests novel modes of signaling by glutamate
- Molecular cloning of a putative vesicular transporter for acetylcholine.
- Molecular cloning and functional analysis of SUT-1, a sulfate transporter from human high endothelial venules
- Molecular cloning and characterization of the human A3 adenosine receptor.
