Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate

AUTOR(ES)

Braunig, Patricia, Rosa, Rafael Diego da, Seibert, Caroline Heidrich, Borsa, Mariana, Stoco, Patricia Hermes, Grisard, Edmundo Carlos, Pinto, Aguinaldo Roberto

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2011-04

RESUMO

In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.

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