Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.

AUTOR(ES)

Gunaratne, P H

RESUMO

Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.

Documentos Relacionados

• Germ-Line Effects of a Mutator, Mu2, in Drosophila Melanogaster
• Viability of Female Germ-Line Cells Homozygous for Zygotic Lethals in DROSOPHILA MELANOGASTER
• Genetic and molecular analysis of chlorambucil-induced germ-line mutations in the mouse.
• Germ-line immortality
• Germ-Line and Somatic Recombination Induced by in Vitro Modified P Elements in Drosophila Melanogaster