Molecular cloning of the 3'-proximal third of Sendai virus genome.
AUTOR(ES)
Dowling, P C
RESUMO
Portions of the Sendai virus genome were randomly cloned by using virion 50S RNA and calf thymus DNA pentanucleotides as primers. The recombinant clones were probed first with radiolabeled products of an in vitro virion RNA polymerase reaction to locate early message clones and then with a probe from the viral genome 3' end to locate the most 3'-proximal clones. Clones were then ordered from the 3' end of the genome and used to construct a genetic map of the 3'-proximal third of the genome by hybrid-selection of mRNAs. We report that the gene order for this region is 3'-NP - P + C - M-5' and that the genetic loci of the viral P and C proteins cannot be separated by these techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=384222Documentos Relacionados
- A Primary Determinant of Cap-Independent Translation Is Located in the 3′-Proximal Region of the Tomato Bushy Stunt Virus Genome
- Molecular characterization of the 3' terminus of the simian hemorrhagic fever virus genome.
- Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome.
- Molecular cloning and physical mapping of the tupaia herpesvirus genome.
- Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome.