Molecular Probes of Spermatozoan Structures
AUTOR(ES)
Edelman, Gerald M.
RESUMO
Several methods have been devised for the isolation and labeling of structural components of spermatozoa. Rodent spermatozoa were cleaved rapidly and specifically at the junction of the heads and tails by treatment with various proteases, and the separate components were isolated by density-gradient centrifugation. Treatment with reducing agents released the mitochondrial membranes from the midpiece, exposing the underlying tail structures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389438Documentos Relacionados
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