Molecular resolution imaging of macromolecular crystals by atomic force microscopy.
AUTOR(ES)
Kuznetsov YuG
RESUMO
Atomic force microscopy (AFM) images at the molecular level have been obtained for a number of different protein and virus crystals. They can be utilized in some special cases to obtain information useful to crystal structure analyses by x-ray diffraction. In particular, questions of space group enantiomer, the packing of molecules within a unit cell, the number of molecules per asymmetric unit, and the dispositions of multiple molecules within the asymmetric unit may be resolved. In addition, because of the increasing sensitivity and resolution of the AFM technique, some molecular features of very large asymmetric units may be within reach. We describe here high-resolution studies, using AFM, to visualize individual molecules and viruses in their crystal lattices. These investigations included fungal lipase, lysozyme, thaumatin, canavalin, and satellite tobacco mosaic virus (STMV).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1184431Documentos Relacionados
- Viscoelasticity of living cells allows high resolution imaging by tapping mode atomic force microscopy.
- High resolution mapping DNAs by R-loop atomic force microscopy.
- Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy.
- Imaging of the membrane surface of MDCK cells by atomic force microscopy.
- Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.