Morfogenese em acetabularia calyculus (Quoy e Gaimard) : fatores relacionados com a diferenciaçãodo chapeu

AUTOR(ES)
DATA DE PUBLICAÇÃO

1990

RESUMO

Morphological variations in the nucleus of the marine algae Acetabularla calyculus (Quoy e Gaimard) were analysed by the use of the fluorescent dye DAPI in cella from different atages of growth in a synchronized culture. The results indicated that the increase in the nuclear volume correaponded to a great development of nucleoli during the cell growth. In the stage before to cap formation (12 mm length) the nucleoli occupied almost 80% of the nucleus, presenting characteriatic structures known as "sausage-like shapes" ; indicating that there is abundant synthesis of rRNA precursors in this stage. At the same time, the chromosomes were unpacked, uncondensed, similar to lampbrush chromosomes, as we observed a high mRNA synthesis in this fase. These results were confirmed by the ultrastructural observations of growing cell tips and cell tipe in the beginning of cap differentiation. Endoplasmatic reticulum membrane-attached ribosomes structures, free ribosomea, Golgi apparatus, secretion vesicles, chloroplasts and mitochondria were visualized at this stage indiçating a high cellular methabolism, resulting in the intense protein synthesis. The cap differentiation is related to the formation of new cellular walls. For that to happen there is a need for an intense production of precursor substances of the cell wall. The ultrastructural observations of cell tips at the beginning of cap differentiation showed the presence of secretion vesicles at the sites of formation of new cell wall. These precursor substances for new cell wall synthesis, produced in the endoplasmatic reticulum and Golgi apparatus, were probably being transported by means of secretion vesicles. The analysis of the radioactive [3H]-uridine RNA?s synthetised in the nucleus and transported along the cell body presented further support for the ultrastructural observations.

ASSUNTO(S)

alga marinha - morfologia morfogenese citologia

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