Morphological and genetic effects of benomyl on polyploid brewing yeasts: isolation of auxotrophic mutants.
AUTOR(ES)
Bilinski, C A
RESUMO
An enrichment procedure after ethyl methanesulfonate mutagenesis and exposure to the fungicide benomyl yielded mutants auxotrophic for several amino acids from two polyploid Saccharomyces spp. Benomyl treatment was found to have a marked morphological effect on polyploid Saccharomyces cerevisiae, causing cells to adopt a characteristic doublet cell morphology in which buds are nearly as large as the parent cells. Experiments in which nuclear division was monitored in benomyl-induced doublet cells by Giemsa nuclear staining demonstrated an unusual sequence of cytological events which culminated in the formation of binucleate parental and mononucleate bud components. The frequency of formation of doublet and binucleate parent cells was found to depend on the strain employed and the benomyl concentration administered.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241619Documentos Relacionados
- Isolation of Candida tropicalis auxotrophic mutants.
- Isolation and genetic characterization of ethanol-resistant reovirus mutants.
- Intracytoplasmic growth and virulence of Listeria monocytogenes auxotrophic mutants.
- Isolation and preliminary characterization of auxotrophic and morphological mutants of the yeastlike form of Paracoccidioides brasiliensis.
- Isolation and genetic characterizations of Bacillus megaterium cobalamin biosynthesis-deficient mutants.