Morphological transformation of established rodent cell lines by high-level expression of the adenovirus type 2 E1a gene.
AUTOR(ES)
Senear, A W
RESUMO
When a strong promoter derived from the mouse metallothionein gene was substituted for the homologous adenovirus type 2 E1a promoter, leading to enhanced levels of E1a RNAs and proteins in cells transfected with the chimeric gene, the E1a gene alone was able to induce in established cell lines alterations in cellular morphology and growth properties similar to those produced by the combined action of E1a and E1b genes. The qualitative effects of E1a gene expression upon cellular properties thus depend on the level of expression of the E1a gene. Furthermore, E1a may be the primary transforming gene of adenoviruses, since it produced many of the characteristics of transformed cells that had previously been attributed to E1b.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367637Documentos Relacionados
- A region in the coding sequence is required for high-level expression of murine histone H3 gene.
- The efficiency of adenovirus transformation of rodent cells is inversely related to the rate of viral E1A gene expression.
- Novel Tat-Encoding Bicistronic Human Immunodeficiency Virus Type 1-Based Gene Transfer Vectors for High-Level Transgene Expression
- High-level eucaryotic in vivo expression of biologically active measles virus hemagglutinin by using an adenovirus type 5 helper-free vector system.
- High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.