Morphometric studies of the skin lymphocytic infiltration on immunohistochemical preparation / Estudo morfometrico das infiltrações linfocitarias cutaneas em preparados imunoistoquimicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Mycosis fungoides (MF) represents the most common skin lymphoid neoplasm. In initial stages, differential diagnosis of MF with other benign dermal lymphoid infiltrates (BDLI) may be impossible on morphological basis alone. In previous studies conducted by our group, only loss of immunoexpression of CD7 in MF proved to be of diagnostic help, but not the ratio between immunoexpression of CD4 and CD8. In the present study, 30 cases of MF and 11 cases of BDLI were analyzed, in order to compare morphometric parameters, which could be of diagnostic aid. As CD7 is frequently deleted in MF, immunohistochemical detection of T-cells was made using an antibody to CD3. Images of 100 CD3-positive cells per case in both groups were captured and analyzed using a simple computer program (Imagelab®) for nuclear perimeter (P), area (A), diameters (D) and nuclear contour index (NCI). All parameters showed statistically significant higher values for MF. Area was the variable with the strongest discriminating power between the two groups of patients. There was a tendency to a correlation between the loss of expression of CD7 and increase in nuclear area. Even if morphologic evaluation is not accurate to distinguish benign versus malignant dermal lymphoid infiltrates, due to the variability of size and shape of these cells, a more sensitive method promptly shows this difference. Our results suggest that morphometry of CD3-positive lymphoid cells may add valuable information in the differential diagnosis of MF and benign dermatoses.

ASSUNTO(S)

imunohistoquimica lymphoma morphometry linfoma morfometria immunohistochemistry

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