Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system.
AUTOR(ES)
Jasin, M
RESUMO
Mouse embryonic stem (ES) cells were compared to COS1 and CV1 cells for their ability to perform extrachromosomal homologous recombination. RSVCAT plasmid substrates consisting of overlapping chloramphenicol acetyltransferase (CAT) gene fragments were transiently transfected into cells and extracts were assayed for CAT activity. Approximately 10% activity, relative to transfection with a complete CAT gene, was recovered for the recombination substrates in each of the cell lines tested. ES cells, therefore, as other cell lines, are capable of high levels of extrachromosomal recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332558Documentos Relacionados
- Rapid assay for extrachromosomal homologous recombination in monkey cells.
- Inactivating the beta 2-microglobulin locus in mouse embryonic stem cells by homologous recombination.
- Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.
- Inducible site-directed recombination in mouse embryonic stem cells.
- Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.