Movement of yeast cortical actin cytoskeleton visualized in vivo.
AUTOR(ES)
Doyle, T
RESUMO
Fusion proteins between the green fluorescent protein (GFP) and the cytoskeleton proteins Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces cerevisiae) localize to the cortical actin patches. The actin fusions could not function as the sole actin source in yeast, but fusions between the actin-binding proteins Abp1p and Sac6p complement fully the phenotypes associated with their gene deletions. Direct observation in vivo reveals that the actin cortical patches move. Movement of actin patches is constrained to the asymmetric distribution of the patches in growing cells, and this movement is greatly reduced when metabolic inhibitors such as sodium azide are added. Fusion protein-labeled patches are normally distributed during the yeast cell cycle and during mating. In vivo observation made possible the visualization of actin patches during sporulation as well.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39454Documentos Relacionados
- Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo.
- Sla2p Is Associated with the Yeast Cortical Actin Cytoskeleton via Redundant Localization Signals
- A yeast TCP-1-like protein is required for actin function in vivo.
- TOR2 is required for organization of the actin cytoskeleton in yeast
- Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo.