Multiple and Specific Initiation of T4 DNA Replication
AUTOR(ES)
Howe, Chin C.
RESUMO
Partially replicated T4 DNA molecules (PRM) whose parental or progeny DNA was labeled with bromodeoxyuridine BUdR was analyzed by gradual shearing followed by CsCl banding of the sheared product. Analysis of PRM containing 18-μm replicated DNA showed that each replicated region was 3- to 6-μm long, indicating three to 6 replicative sites per molecule. Analysis of PRM containing 9-μm replicated DNA similarly indicated two to three replicated regions per molecule. DNA from the replicated regions of PRM containing 10-μm replicated DNA (“donor”) was hybridized to DNA from mature phage (“recipient”), and the resulting hybrid was subjected to digestion with exonuclease I. The extent of protection of the recipient and more efficient self-annealing of progeny fragments from PRM indicated that the replicated regions represented 8 to 10 nonrandom locations of the genome. Possible significance of multiple sites for initiation of DNA replication is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355239Documentos Relacionados
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