Multiple mRNAs for human alcohol dehydrogenase (ADH): developmental and tissue specific differences.
AUTOR(ES)
Bilanchone, V
RESUMO
Human class I alcohol dehydrogenase (ADH) genes show developmental and tissue specific differences in expression at the polypeptide level. In these studies ADH expression was investigated at the RNA level. Northern blot analysis of total and poly (A) RNA from adult liver using pADH12 probe demonstrated multiple RNA size classes of 2.6, 2.2, 1.9 and 1.6kb. In contrast, fetal liver, and fetal intestine contained only 2.6 and 1.6kb mRNA while fetal lung showed only 2.6kb mRNA. All of these tissues showed a relative reduction in the amount of ADH mRNA present when compared to adult liver. Immunoprecipitation of in vitro translation products of adult liver RNA by polyclonal ADH antibody revealed a single polypeptide of 40,000 daltons. This result points out the homogeneity of size of class I ADH polypeptides despite mRNA size diversity. Variation in length of the 3' untranslated region probably contributes to the multiple size classes of ADH mRNA observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339824Documentos Relacionados
- Tissue-specific expression of mRNAs for dipeptidyl carboxypeptidase isoenzymes.
- A genetic locus regulates the expression of tissue-specific mRNAs from multiple transcription units.
- The mechanism of production of multiple mRNAs for human glycophorin A.
- Developmental changes in translatable mRNAs for the cerebral enolase isozymes alphaalpha and gammagamma.
- Tissue specific expression of the rat Ah-receptor and ARNT mRNAs.
