Multiple, Tandem Plasmid Integration in Saccharomyces cerevisiae
AUTOR(ES)
Orr-Weaver, Terry L.
RESUMO
Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368590Documentos Relacionados
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