Multiplex PCRs for Identification of Escherichia coli Virulence Genes
AUTOR(ES)
Pass, M. A.
FONTE
American Society for Microbiology
RESUMO
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=86652Documentos Relacionados
- Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli
- Evaluation of Multiplex PCRs for Diagnosis of Infection with Diarrheagenic Escherichia coli and Shigella spp.
- Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli
- Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes.
- Multiplex PCR for Detection of Three Plasmid-Borne Genes of Enteroaggregative Escherichia coli Strains
