Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed
AUTOR(ES)
Das, Mithu, Banerjee, Rintu, Bal, Satish
FONTE
Brazilian Archives of Biology and Technology
DATA DE PUBLICAÇÃO
2008-02
RESUMO
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28ºC temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by ~ 2.3 fold.
Documentos Relacionados
- Effect of Colloidal Materials on Cellulase Production by Trichoderma reesei Rut-C30
- Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction.
- Conditions of formation, purification, and characterization of an alpha-galactosidase of Trichoderma reesei RUT C-30.
- Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei.
- Double-antibody sandwich enzyme-linked immunosorbent assay for quantitation of endoglucanase I of Trichoderma reesei.