Mutants of Escherichia coli B/r Defective in Deoxyribonucleic Acid Initiation: dnaI, a New Gene for Replication
AUTOR(ES)
Beyersmann, D.
RESUMO
Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna− sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC+ and dnaI+ were dominant to dnaC− and dnaI− alleles, respectively. However, dnaA− was dominant to the wild-type allele dnaA+. All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246822Documentos Relacionados
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