Mutations Affecting the Trna-Splicing Endonuclease Activity of Saccharomyces Cerevisiae
AUTOR(ES)
Winey, M.
RESUMO
Two unlinked mutations that alter the enzyme activity of tRNA-splicing endonuclease have been identified in yeast. The sen1-1 mutation, which maps on chromosome 12, causes temperature-sensitive growth, reduced in vitro endonuclease activity, and in vivo accumulation of unspliced pre-tRNAs. The sen2-1 mutation does not confer a detectable growth defect, but causes a temperature-dependent reduction of in vitro endonuclease activity. Pre-tRNAs do not accumulate in sen2-1 strains. The in vitro enzyme activities of sen1-1 and sen2-1 complement in extracts from a heterozygous diploid, but fail to complement in mixed extracts from separate sen1-1 and sen2-1 haploid strains. These results suggest a direct role for SEN gene products in the enzymatic removal of introns from tRNA that is distinct from the role of other products known to affect tRNA splicing.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1203316Documentos Relacionados
- SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiae.
- Substrate recognition and identification of splice sites by the tRNA-splicing endonuclease and ligase from Saccharomyces cerevisiae.
- SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing.
- Intron mutations affect splicing of Saccharomyces cerevisiae SUP53 precursor tRNA.
- Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae.
