Mutations in Gsf1 and Gsf2 Alter Glucose Signaling in Saccharomyces Cerevisiae

AUTOR(ES)

Sherwood, P. W.

RESUMO

One function of the Saccharomyces cerevisiae Snf1 protein kinase is to relieve glucose repression of SUC, GAL, and other genes in response to glucose depletion. To identify genes that regulate Snf1 kinase activity, we have selected mutants that inappropriately express a SUC2promoter::HIS3 gene fusion when grown in glucose and that require Snf1 function for this phenotype. Mutations representing two new complementation groups (gsf1 and gsf2) were isolated. gsf1 mutations affect two distinct responses to glucose: the Snf1-regulated glucose repression of SUC2 and GAL10 transcription and the Snf1-independent induction by glucose of HXT1 transcription. gsf2 mutations relieve glucose repression of SUC2 and GAL10 transcription and, in combination with snf1Δ, cause an extreme slow growth phenotype. The GSF2 gene was cloned by complementation of the gsf2-1 snf1Δ slow growth phenotype and encodes a previously uncharacterized 46 kD protein.

Documentos Relacionados

• Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I
• G protein mutations that alter the pheromone response in Saccharomyces cerevisiae.
• Synergistic Release from Glucose Repression by Mig1 and Ssn Mutations in Saccharomyces Cerevisiae
• Mutations releasing mitochondrial biogenesis from glucose repression in Saccharomyces cerevisiae.
• Trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes.