Necessary Conditions for Isolation of Tightly Coupled Higher Plant Mitochondria 1
AUTOR(ES)
Ikuma, Hiroshi
RESUMO
A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mitochondria include: (a) gentle tissue disruption; (b) rigorous exclusion of contaminating particles from the mitochondrial fraction; (c) the use of an optimal grinding medium containing a high concentration of mannitol, 10 mm phosphate or triethanolamine buffer to maintain the pH above 7.2, ethylenediaminetetraacetic acid at 1 mm level, cysteine at a low concentration (0-5 mm), and a high concentration of bovine serum albumin (0.1-1.0%); and (d) the use of the proper wash and suspending medium consisting of mannitol, buffer, and bovine serum albumin at the same concentration as in the grinding medium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=396510Documentos Relacionados
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