Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins.

AUTOR(ES)

Ordway, R W

RESUMO

Several lines of investigation have now converged to indicate that the neurotransmitter release apparatus is formed by assembly of cytosolic proteins with proteins of the synaptic vesicle and presynaptic terminal membranes. We are undertaking a genetic approach in Drosophila melanogaster to investigate the functions of two types of cytosolic proteins thought to function in this complex: N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs). We have identified Drosophila homologs of the vertebrate and yeast NSF and SNAP genes. Both Drosophila genes encode polypeptides that closely resemble their vertebrate counterparts and are expressed in the nervous system; neither appears to be in a family of closely related Drosophila genes. These results indicate that the Drosophila NSF and SNAP genes are excellent candidates for mutational analysis of neurotransmitter release.

Documentos Relacionados

• Molecular characterization of two Brassica napus pollen-expressed genes encoding putative arabinogalactan proteins.
• Identification and characterization of functional genes encoding the mouse major urinary proteins.
• Characterization and mapping of human genes encoding zinc finger proteins.
• Characterization of Secretory Genes ypt1/yptA and nsf1/nsfA from Two Filamentous Fungi: Induction of Secretory Pathway Genes of Trichoderma reesei under Secretion Stress Conditions
• Isolation and restriction site maps of the genes encoding five Mycobacterium tuberculosis proteins.